QIAseq Targeted Panel Analysis

Accurately Identify Genetic Variants with Ease.

About Targeted Panel Analysis

Biomedical Genomics Workbench 5.x and the QIAseq Targeted Panel Analysis plugin provide in combination a true Sample to Insight Solution for targeted variant detection in both somatic and germline applications. The plugin also supports the analysis of Illumina and Ion Torrent sequencing data.

The QIAseq Targeted Panel Analysis plugin is a one-stop solution where you can select the QIAseq Targeted DNA, QIAseq RNAscan, or QIAseq RNA panel of choice and launch built-in analysis workflows tailored to the respective panel, application, and sequencing platform.

The plugin combines state-of-the-art molecular biology with accurate and computationally efficient variant detection. The analysis can be run locally on different operating systems, on a server or high-performance cluster, or in a virtual private cloud (VPC). This ensures flexibility, scalability, and data security, all of which is necessary in advanced clinical research applications.

 

Detect Any Variant

Building on the well-established QIAGEN CLC solutions, the QIAseq Targeted Panels Analysis plugin is capable of detecting many different types of variants.

These include:

  • Single Nucleotide Variants (SNVs)
  • Multiple Nucleotide Variants (MNVs)
  • Inserts and deletions (InDels)
  • Gene Fusions
  • Copy Number Variants (CNVs)

For more details, download the Application Notes.

 

QIAseq™ Targeted Panels: Accurately Identify Genetic Variants with Ease  |  DOWNLOAD NOW

A Sample to Insight® NGS Solution for Myeloid Neoplasms: Redefined Amplicon Sequencing for Low Variant Detection and Interpretation  |  DOWNLOAD NOW

 

Analyzing QIAseq Data Made Easy

The QIAseq Targeted Panel Analysis plugin includes the user-friendly QIAseq Panel Analysis interface, which guides you through all necessary steps for analyzing QIAseq Targeted DNA, RNAscan and RNA panel data. Integration with Ingenuity Variant Analysis provides valuable clinical insights to identified variants.

For data analysis, the plugin relies on preconfigured, ready-to-use workflows. The workflow system in QIAGEN’s CLC Workbenches combines ease-of-use with reproducibility. While analysis parameters and conditions are preconfigured and optimized for the respective panel type, you can easily duplicate and modify workflows, adapting them to other sequencing applications. Of course, custom parameters and settings can be locked again to ensure reproducibility, and the parameter settings used for a given analysis are recorded. Reported variants are visualized in an intuitive and interactive manner, to guide validation or additional analyses.

 

Increased Variant Calling Accuracy via Unique Molecular Indices and Advanced Bioinformatics

NGS library preparation using QIAseq chemistry involves Unique Molecular Indices (UMIs), also known as molecular barcodes. This technique reduces systematic biases and errors from the amplification and NGS processes.

Using the information provided by UMIs, reads can be assigned to individual captured DNA or RNA molecules, allowing for a computational correction of amplification bias and sequencing error. Through the use of UMIs and the advanced algorithms for the correction of sequencing and replication error in the QIAseq Targeted Panel Analysis plugin, the NGS error rate is greatly reduced. Indeed, for high coverage sequencing applications, such as in liquid biopsy, UMIs can markedly increase the overall accuracy of the variant calling in terms of both sensitivity and precision.

This combination of molecular biology and smart bioinformatics leads to more accurate reporting of variant frequency or expression level estimates.

This module now supports the following 33 panels:

QIAseq Targeted DNA Panels
DHS-001Z Human Breast Cancer Panel
DHS-002Z Human Colorectal Cancer Panel
DHS-003Z Human Myeloid Neoplasms Panel
DHS-005Z Human Lung Cancer Panel
DHS-104Z Human Pharmacogenomics Panel
DHS-3011Z Human Inherited Disease Panel
DHS-3501Z Human Comprehensive Cancer Panel
DHS-101Z Human Actionable Solid Tumor Panel
DHS-102Z Human BRCA1 and BRCA2 Panel
DHS-103Z Human BRCA1 and BRCA2 Plus Panel
DHS-105Z Human Mitochondria Panel
 

QIAseq Targeted

 

RNAscan Panels

FHS-001Z Human Leukemia Panel
FHS-002Z Human Solid Tumor Panel
FHS-003Z Human Lung Cancer Panel
FHS-004Z Human Oncology Panel
 

QIAseq Targeted

 

RNA Panels

RHS-001Z Human Angiogenesis and Endothelial Cell Biology
RHS-002Z Human Apoptosis and Cell Death
RHS-003Z Human Cancer Transcriptome
RHS-004Z Human Extracellular Matrix and Cell Adhesion Molecules
RHS-005Z Human Inflammation and Immunity Transcriptome
RHS-006Z Human Molecular Toxicology Transcriptome
RHS-007Z Human Signal Transduction PathwayFinder
RHS-008Z Human Stem Cell and Differentiation Markers
RHS-009Z Human Immuno-Oncology
RMM-001Z Mouse Angiogenesis and Endothelial Cell Biology
RMM-002Z Mouse Apoptosis and Cell Death
RMM-003Z Mouse Cancer Transcriptome
RMM-004Z Mouse Extracellular Matrix and Cell Adhesion Molecules
RMM-005Z Mouse Inflammation and Immunity Transcriptome
RMM-006Z Mouse Molecular Toxicology Transcriptome
RMM-007Z Mouse Signal Transduction PathwayFinder
RMM-008Z Mouse Stem Cell and Differentiation Markers
RMM-009Z Mouse Immuno-Oncology

To get a complete overview of the improvements in the latest version, please refer to the Latest Improvements listings.

For more details, download the Application Notes.

QIAseq™ Targeted Panels: Accurately Identify Genetic Variants with Ease  |  DOWNLOAD NOW

A Sample to Insight® NGS Solution for Myeloid Neoplasms: Redefined Amplicon Sequencing for Low Variant Detection and Interpretation  |  DOWNLOAD NOW

Downloads


Plugin Manual

Download manual

Plugin Download

Download plugin

Download QIAseq Targeted Panel Analysis


Version

Platform support

Download

1.1.0

Biomedical Genomics Workbench


 [5.0.1]

1.0.1

Biomedical Genomics Workbench


 [5.0.0]

Server plugin Downloads

Download plugin

Download QIAseq Targeted Panel Analysis Server Plugin


Version

Platform support

Download

1.1.0

Biomedical Genomics Server Solution


 [10.0.1]

1.0.1

Biomedical Genomics Server Solution


 [10.0]

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