Biomedical Genomics Workbench 5.x and the QIAseq Targeted Panel Analysis plugin provide in combination a true Sample to Insight Solution for targeted variant detection in both somatic and germline applications. The plugin also supports the analysis of Illumina and Ion Torrent sequencing data.
The QIAseq Targeted Panel Analysis plugin is a one-stop solution where you can select the QIAseq Targeted DNA, QIAseq RNAscan, or QIAseq RNA panel of choice and launch built-in analysis workflows tailored to the respective panel, application, and sequencing platform.
The plugin combines state-of-the-art molecular biology with accurate and computationally efficient variant detection. The analysis can be run locally on different operating systems, on a server or high-performance cluster, or in a virtual private cloud (VPC). This ensures flexibility, scalability, and data security, all of which is necessary in advanced clinical research applications.
Detect Any Variant
Building on the well-established QIAGEN CLC solutions, the QIAseq Targeted Panels Analysis plugin is capable of detecting many different types of variants.
Learn more about how we detect these variants by reading the Application Note.
Analyzing QIAseq Data Made Easy
The QIAseq Targeted Panel Analysis plugin includes the user-friendly QIAseq Panel Analysis interface, which guides you through all necessary steps for analyzing QIAseq Targeted DNA, RNAscan and RNA panel data. Integration with Ingenuity Variant Analysis provides valuable clinical insights to identified variants.
For data analysis, the plugin relies on preconfigured, ready-to-use workflows. The workflow system in QIAGEN’s CLC Workbenches combines ease-of-use with reproducibility. While analysis parameters and conditions are preconfigured and optimized for the respective panel type, you can easily duplicate and modify workflows, adapting them to other sequencing applications. Of course, custom parameters and settings can be locked again to ensure reproducibility, and the parameter settings used for a given analysis are recorded. Reported variants are visualized in an intuitive and interactive manner, to guide validation or additional analyses.
Increased Variant Calling Accuracy via Unique Molecular Indices and Advanced Bioinformatics
NGS library preparation using QIAseq chemistry involves Unique Molecular Indices (UMIs), also known as molecular barcodes. This technique reduces systematic biases and errors from the amplification and NGS processes.
Using the information provided by UMIs, reads can be assigned to individual captured DNA or RNA molecules, allowing for a computational correction of amplification bias and sequencing error. Through the use of UMIs and the advanced algorithms for the correction of sequencing and replication error in the QIAseq Targeted Panel Analysis plugin, the NGS error rate is greatly reduced. Indeed, for high coverage sequencing applications, such as in liquid biopsy, UMIs can markedly increase the overall accuracy of the variant calling in terms of both sensitivity and precision.
This combination of molecular biology and smart bioinformatics leads to more accurate reporting of variant frequency or expression level estimates.
This module now supports the following 33 panels:
|QIAseq Targeted||DNA Panels|
|DHS-001Z||Human Breast Cancer Panel|
|DHS-002Z||Human Colorectal Cancer Panel|
|DHS-003Z||Human Myeloid Neoplasms Panel|
|DHS-005Z||Human Lung Cancer Panel|
|DHS-104Z||Human Pharmacogenomics Panel|
|DHS-3011Z||Human Inherited Disease Panel|
|DHS-3501Z||Human Comprehensive Cancer Panel|
|DHS-101Z||Human Actionable Solid Tumor Panel|
|DHS-102Z||Human BRCA1 and BRCA2 Panel|
|DHS-103Z||Human BRCA1 and BRCA2 Plus Panel|
|DHS-105Z||Human Mitochondria Panel|
|FHS-001Z||Human Leukemia Panel|
|FHS-002Z||Human Solid Tumor Panel|
|FHS-003Z||Human Lung Cancer Panel|
|FHS-004Z||Human Oncology Panel|
|RHS-001Z||Human Angiogenesis and Endothelial Cell Biology|
|RHS-002Z||Human Apoptosis and Cell Death|
|RHS-003Z||Human Cancer Transcriptome|
|RHS-004Z||Human Extracellular Matrix and Cell Adhesion Molecules|
|RHS-005Z||Human Inflammation and Immunity Transcriptome|
|RHS-006Z||Human Molecular Toxicology Transcriptome|
|RHS-007Z||Human Signal Transduction PathwayFinder|
|RHS-008Z||Human Stem Cell and Differentiation Markers|
|RMM-001Z||Mouse Angiogenesis and Endothelial Cell Biology|
|RMM-002Z||Mouse Apoptosis and Cell Death|
|RMM-003Z||Mouse Cancer Transcriptome|
|RMM-004Z||Mouse Extracellular Matrix and Cell Adhesion Molecules|
|RMM-005Z||Mouse Inflammation and Immunity Transcriptome|
|RMM-006Z||Mouse Molecular Toxicology Transcriptome|
|RMM-007Z||Mouse Signal Transduction PathwayFinder|
|RMM-008Z||Mouse Stem Cell and Differentiation Markers|
To get a complete overview of the improvements in the latest version, please refer to the Latest Improvements listings.
For more details, download the Application Note.
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