The method uses local sequence alignments (BLAST) to find similar sequences (potential homologs) for one or several input sequences. The program extracts all GO terms associated to each of the obtained hits and returns an evaluated GO annotation for the query sequence(s). Enzyme codes are obtained by mapping from equivalent GOs while InterPro motifs can directly be queried at the InterProScan web service. A basic annotation process with Blast2GO PRO consists of 3 steps: blasting, mapping, and annotation. These steps are described in the manual including further explanations and information on additional functions.
Application note: Whole genome functional annotation of Solanum lycopersicum
For further information, please visit the Blast2GO website.
A. Conesa, S. Götz, J. M. Garcia-Gomez, J. Terol, M. Talon and M. Robles. “Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research”, Bioinformatics, Vol. 21, September, 2005, pp. 3674-3676.
A. Conesa and S. Götz. “Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics”, International Journal of Plant Genomics, Vol. 2008. 2008, pp. 1-13.
S. Götz et al. “High-throughput functional annotation and data mining with the Blast2GO suite”, Nucleic Acids Research, Vol. 36, June, 2008, pp. 3420-3435.
S. Götz et al. “B2G-FAR, a species centered GO annotation repository”, Bioinformatics, Vol. 27 (7), 2011, pp. 919-924. (675 characters)