Bisulfite Sequencing

Cytosine methylation is probably the best studied form of epigenetic regulation of gene expression. The Bisulfite Sequencing plugin offers an all-in-one solution to common problems that scientists face when studying cytosine methylation data:

  • The plugin reveals methylated cytosines genome wide and at single base level resolution
  • Users can compare cytosine methylation between samples and identify genomic regions where methylation levels differ
  • Support for reduced representation sequencing. The analysis can be focused on targeted regions expected to methylated
  • Included statistical tools allow sample comparison and accommodate different experimental designs. Results are easy to interpret and exportable

All functionalities required for comprehensive bisulfite sequencing analysis are split into three user-friendly tools, which can be combined into an automated workflow.

Tutorials

PDF tutorial is available here.

Latest improvements

We frequently release updates and improvements such as bug fixes or new features. To get a complete overview, please visit the latest improvements page.

 

Downloads


Plugin Manual

Download manual

Plugin Download

Download plugin

Download Bisulfite Sequencing


Version

Platform support

Download

2.0.0

Biomedical Genomics Workbench


 [4.1.1, 4.1.0, 4.0]

CLC Genomics Workbench


 [10.1.1, 10.1.0, 10.0.1, 10.0]

1.1.1

Biomedical Genomics Workbench


 [3.5.4, 3.5.3, 3.5.2, 3.5.1, 3.5, 3.0.1, 3.0]

CLC Genomics Workbench


 [9.5.4, 9.5.3, 9.5.2, 9.5.1, 9.5, 9.0.1, 9.0]

1.0

Biomedical Genomics Workbench


 [2.5.4, 2.5.3, 2.5.2, 2.5.1, 2.5]

CLC Genomics Workbench


 [8.5.4, 8.5.3, 8.5.2, 8.5.1, 8.5]

Server plugin Downloads

Download plugin

Download Bisulfite Sequencing Server Plugin


Version

Platform support

Download

2.0.0

Biomedical Genomics Server Solution


 [9.1.1, 9.1, 9.0]

CLC Genomics Server


 [9.1.1, 9.1.0, 9.0]

1.1.1

Biomedical Genomics Server Solution


 [8.5.4, 8.5.3, 8.5.2, 8.5.1, 8.5, 8.0.1, 8.0]

CLC Genomics Server


 [8.5.4, 8.5.3, 8.5.2, 8.5.1, 8.5, 8.0.1, 8.0]

1.0

Biomedical Genomics Server Solution


 [7.5.4, 7.5.3, 7.5.2, 7.5.1, 7.5]

CLC Genomics Server


 [7.5.4, 7.5.3, 7.5.2, 7.5.1, 7.5]

Step 1 - About you

Step 2 - Organizational details

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